您可以使用来以这种方式进行操作ctypes，不需要 C中的 辅助应用程序：

rehash.py

#! /usr/bin/env python

''' A resumable implementation of SHA-256 using ctypes with the OpenSSL crypto library

    Written by PM 2Ring 2014.11.13
'''

from ctypes import *

SHA_LBLOCK = 16
SHA256_DIGEST_LENGTH = 32

class SHA256_CTX(Structure):
    _fields_ = [
        ("h", c_long * 8),
        ("Nl", c_long),
        ("Nh", c_long),
        ("data", c_long * SHA_LBLOCK),
        ("num", c_uint),
        ("md_len", c_uint)
    ]

HashBuffType = c_ubyte * SHA256_DIGEST_LENGTH

#crypto = cdll.LoadLibrary("libcrypto.so")
crypto = cdll.LoadLibrary("libeay32.dll" if os.name == "nt" else "libssl.so")

class sha256(object):
    digest_size = SHA256_DIGEST_LENGTH

    def __init__(self, datastr=None):
        self.ctx = SHA256_CTX()
        crypto.SHA256_Init(byref(self.ctx))
        if datastr:
            self.update(datastr)

    def update(self, datastr):
        crypto.SHA256_Update(byref(self.ctx), datastr, c_int(len(datastr)))

    #Clone the current context
    def _copy_ctx(self):
        ctx = SHA256_CTX()
        pointer(ctx)[0] = self.ctx
        return ctx

    def copy(self):
        other = sha256()
        other.ctx = self._copy_ctx()
        return other

    def digest(self):
        #Preserve context in case we get called before hashing is
        # really finished, since SHA256_Final() clears the SHA256_CTX
        ctx = self._copy_ctx()
        hashbuff = HashBuffType()
        crypto.SHA256_Final(hashbuff, byref(self.ctx))
        self.ctx = ctx
        return str(bytearray(hashbuff))

    def hexdigest(self):
        return self.digest().encode('hex')

#Tests
def main():
    import cPickle
    import hashlib

    data = ("Nobody expects ", "the spammish ", "imposition!")

    print "rehash\n"

    shaA = sha256(''.join(data))
    print shaA.hexdigest()
    print repr(shaA.digest())
    print "digest size =", shaA.digest_size
    print

    shaB = sha256()
    shaB.update(data[0])
    print shaB.hexdigest()

    #Test pickling
    sha_pickle = cPickle.dumps(shaB, -1)
    print "Pickle length:", len(sha_pickle)
    shaC = cPickle.loads(sha_pickle)

    shaC.update(data[1])
    print shaC.hexdigest()

    #Test copying. Note that copy can be pickled
    shaD = shaC.copy()

    shaC.update(data[2])
    print shaC.hexdigest()


    #Verify against hashlib.sha256()
    print "\nhashlib\n"

    shaD = hashlib.sha256(''.join(data))
    print shaD.hexdigest()
    print repr(shaD.digest())
    print "digest size =", shaD.digest_size
    print

    shaE = hashlib.sha256(data[0])
    print shaE.hexdigest()

    shaE.update(data[1])
    print shaE.hexdigest()

    #Test copying. Note that hashlib copy can NOT be pickled
    shaF = shaE.copy()
    shaF.update(data[2])
    print shaF.hexdigest()


if __name__ == '__main__':
    main()

resumable_SHA-256.py

#! /usr/bin/env python

''' Resumable SHA-256 hash for large files using the OpenSSL crypto library

    The hashing process may be interrupted by Control-C (SIGINT) or SIGTERM.
    When a signal is received, hashing continues until the end of the
    current chunk, then the current file position, total file size, and
    the sha object is saved to a file. The name of this file is formed by
    appending '.hash' to the name of the file being hashed.

    Just re-run the program to resume hashing. The '.hash' file will be deleted
    once hashing is completed.

    Written by PM 2Ring 2014.11.14
'''

import cPickle as pickle
import os
import signal
import sys

import rehash

quit = False

blocksize = 1<<16   # 64kB
blocksperchunk = 1<<8

chunksize = blocksize * blocksperchunk

def handler(signum, frame):
    global quit
    print "\nGot signal %d, cleaning up." % signum
    quit = True


def do_hash(fname, filesize):
    hashname = fname + '.hash'
    if os.path.exists(hashname):
        with open(hashname, 'rb') as f:
            pos, fsize, sha = pickle.load(f)
        if fsize != filesize:
            print "Error: file size of '%s' doesn't match size recorded in '%s'" % (fname, hashname)
            print "%d != %d. Aborting" % (fsize, filesize)
            exit(1)
    else:
        pos, fsize, sha = 0, filesize, rehash.sha256()

    finished = False
    with open(fname, 'rb') as f:
        f.seek(pos)
        while not (quit or finished):
            for _ in xrange(blocksperchunk):
                block = f.read(blocksize)
                if block == '':
                    finished = True
                    break
                sha.update(block)

            pos += chunksize
            sys.stderr.write(" %6.2f%% of %d\r" % (100.0 * pos / fsize, fsize))
            if finished or quit:
                break

    if quit:
        with open(hashname, 'wb') as f:
            pickle.dump((pos, fsize, sha), f, -1)
    elif os.path.exists(hashname):
        os.remove(hashname)

    return (not quit), pos, sha.hexdigest()


def main():
    if len(sys.argv) != 2:
        print "Resumable SHA-256 hash of a file."
        print "Usage:\npython %s filename\n" % sys.argv[0]
        exit(1)

    fname = sys.argv[1]
    filesize = os.path.getsize(fname)

    signal.signal(signal.SIGINT, handler)
    signal.signal(signal.SIGTERM, handler)

    finished, pos, hexdigest = do_hash(fname, filesize)
    if finished:
        print "%s  %s" % (hexdigest, fname)
    else:
        print "sha-256 hash of '%s' incomplete" % fname
        print "%s" % hexdigest
        print "%d / %d bytes processed." % (pos, filesize)


if __name__ == '__main__':
    main()

演示

import rehash
import pickle
sha=rehash.sha256("Hello ")
s=pickle.dumps(sha.ctx)
sha=rehash.sha256()
sha.ctx=pickle.loads(s)
sha.update("World")
print sha.hexdigest()

输出

a591a6d40bf420404a011733cfb7b190d62c65bf0bcda32b57b277d9ad9f146e

注意：我要感谢PM2Ring的出色代码。